DNase I, RNase free

NA.85

RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation.

Toxicity: Standard Handling (A)

One unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme.

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

liquid

Novagen^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−20℃

1000 u in Glass bottle

10 - Combustible liquids

WGK 1