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Rosetta-gami 2 Competent Cells-Novagen

产品编号:4082105
规格:Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
包装规格:1 ML
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40821051 ML4130
产品性质
Quality Level【质量水平】
100
manufacturer/tradename
Novagen®
storage condition【储存条件】
OK to freeze
cell transformation【细胞转化】
transformation efficiency: 2×106 cfu/μg
storage temp.【储存温度】
−70℃
基本信息
General description【一般描述】
Rosetta-gami 2 host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed in E. coli. These trxB/gor mutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs.
Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] gor522::Tn10 trxB pRARE2 (CamR, StrR, TetR)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
Components【组分】
0.4 ml1 mlComponent

2 × 0.2 ml5 × 0.2 mlRosetta-gami 2 Competent Cells

2 × 2 ml4 × 2 mlSOC Medium

10 µl10 µlTest Plasmid
Warning【警告】
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Legal Information【法律信息】
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
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