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Insect PopCulture® Reagent

产品编号:4082228
规格:Protein extraction directly from insect cell cultures
包装规格:50 ML,250 ML
产品类别:进口试剂
品牌:Millipore
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
408222850 ML2350
4082228250 ML8390
产品性质
Quality Level【质量水平】
100
manufacturer/tradename
Novagen®
storage condition【储存条件】
OK to freeze
shipped in【运输】
ambient
storage temp.【储存温度】
10-30℃
packaging【包装】
250, 50 ml in Plastic ampoule
基本信息
General description【一般描述】
Insect POPCULTURE® Reagent is a detergent-based lysis reagent specifically formulated for total insect cell culture extraction without the need for centrifugation. The improved method recovers both protein released into the medium and intracellular protein, increasing processing efficiency and target protein yields (Loomis 2002). It is amenable for automated expression-level screening and is fully compatible with the Ni-NTA His•Bind<TMSYMBOL></TMSYMBOL> affinity purification method. Insect PopCulture reagent can be used for protein extraction from insect cells grown in suspension and adherent cells grown on tissue culture plates.
Features and Benefits【特点和优势】
  • No need to separate cells from culture media
  • No need to clarify cell extracts prior to purification
  • Higher protein yield due to target protein recovery from both medium and cells
  • Direct affinity adsorption of target proteins to IMAC affinity resin from the total culture extract
  • Compatible with protease inhibitor cocktails
  • Ideal for high-throughput, expression-level screening and protein purification
  • Compatible with transfected and baculovirus-infected insect cell cultures
Warning【警告】
Toxicity: Irritant (B)
Other Notes【其他说明】
Loomis, K., et al. 2002. inNovations15, 16.
Legal Information【法律信息】
This product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
POPCULTURE is a registered trademark of Merck KGaA, Darmstadt, Germany
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
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