Anti-Bromodeoxyuridine-Fluorescein

100

mouse

fluorescein conjugate

purified immunoglobulin

primary antibodies

BMC9318, monoclonal

≥90% (HPLC and SDS-PAGE)

solution

pkg of 50 μg (500 μl)

Roche

flow cytometry: suitable
immunohistochemistry: suitable

IgG1

dry ice

−20℃

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Anti-bromodeoxyuridine is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells undergoing replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, antibromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously.
Monoclonal antibody to 5-bromo-2′-deoxyuridine-5′-monophosphate conjugated to fluorescein.

The antibody specifically binds to bromodeoxyuridine and crossreacts with iodouridine (10%). Antibromo- deoxyuridine does not crossreact with fluorodeoxy- uridine, nor with any endogenous cellular components such as thymidine or uridine.

Apparently, the epitope is inside of the DNA helix. DNA has to be denatured to ssDNA before antibody efficiently binds to DNA-BrdU.

Solution, stabilized with phosphate buffered saline, pH 7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) gelatin.

Working concentration: Flow cytometry: 0.5 μg/100 μl (106 cells); Immunohistocytochemistry: 50 μg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.

Anti-Bromodeoxyuridine shows 10% cross reaction with iodo-deoxyuridine, but no cross reaction to fluoro-deoxyuridine.
No cross reaction to any endogenous thymidine or uridine.
Cross reactivity with 5-Br-UTP has not been tested but it is suggested that there is a good chance for reaction, because the only difference is an absent hydroxyl group on the ribose distal to the bromine substitution.

仅用于生命科学研究。不可用于诊断。

12 - Non Combustible Liquids

nwg