Anti-Hsp90α Mouse mAb (EMD-17D7)

100

mouse

purified antibody

primary antibodies

EMD-17D7, monoclonal

liquid

≤0.1% sodium azide as preservative

human

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG1

wet ice

−20℃

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

This Anti-Hsp90α Mouse mAb (EMD-17D7) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Hsp90α.
Recognizes the ~90 kDa Hsp90α protein in HeLa cells. Does not recognize Hsp90β.
Protein G PLUS/protein A purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90α protein.

Hsp90α, His•Tag, Human, Recombinant (Cat. No. 385901)
Human

ELISA (see comments)
Immunoblotting (1 g/ml)
Immunoprecipitation (5 g/0.7 mg protein)

Toxicity: Irritant (B)

In PBS.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
H460 and HeLa cells

Scheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA96, 1297.
Nemoto, T., et al. 1997 J. Biol. Chem.272, 26179.
Nemoto, T., et al. 1998. Biochem. J.330, 989.
Detects native and recombinant Hsp90α. Does not recognize Hsp90β. This antibody will also work for ELISA, but concentration is assay dependent. Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

10 - Combustible liquids

WGK 1