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产品别名
PKG, Iα, Bovine Lung
cGMP-Dependent Protein Kinase Catalytic Subunit, PKG Iα Catalytic Subunit, Protein Kinase G Iα
cGMP-Dependent Protein Kinase Catalytic Subunit, PKGIα Catalytic Subunit, Protein Kinase G Iα
产品性质
| form【形式】 | liquid |
| Quality Level【质量水平】 | 100 |
| Specific Activity | ≥1300 units/μg protein |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −70℃ |
| packaging【包装】 | 4 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
| General description【一般描述】 | Native PKGIα purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu. Note: 4µg = 6000 units. |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Unit Definition【单位定义】 | One unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C. |
| Physical form【外形】 | In 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8. |
| Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-70°C). |
| Other Notes【其他说明】 | Brophy, M.C., et al. 2006. J. Vasc. Res.39, 95. Colbran J.L., et al. 1992. J. Biol. Chem.267, 9589. Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem258, 11391. Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem66, 253. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Pictograms【象形图】 | GHS07 |
| Signal word【警示用语：】 | Warning |
| Hazard Statements | H317 |
| Precautionary Statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| Hazard Classifications【危险分类】 | Skin Sens. 1 |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 2 |
