Anti-CD39 Antibody, clone 5F2

32160702

NA.41

Ectonucleoside triphosphate diphosphohydrolase 1 (NTDase 1 or CD39) belongs to the membrane-bound ecto-nucleoside triphosphates diphosphohydrolase family (E-NTPDases) that are characterized for their ability to hydrolyze nucleoside di- and triphosphates, such as ATP, in the presence of cations, i.e. CA2+ and Mg2+. CD39 is an important membrane protein containing two transmembrane domains and a large NTPDase active, extracellular region containing apyrase conserved regions. It is heavily glycosolated, having six known N-glycosolation sites, and functions on the plasma membrane’s outer surface. CD39 initiates the metabolism, of ATP and ADP to adenosine through a 2-step exzymatic reaction, providing extracellular adenosine for the purpose of regulating various inflammatory endpoints. It is a putative marker for B-cell activation and its expression has also been observed on NK-cells, dendritic cells, T-cells, and vascular endothelial cells. CD39 is a known inhibitor of ADP-induced platelet aggregation and may also play a role in angiogenesis.

This antibody recognizes CD39.

Recombinant protein corresponding to mouse CD39.

This Anti-CD39 Antibody, clone 5F2 is validated for use in FC, WB for the detection of CD39.
Western Blot Analysis: A previous lot was used by an independent laboratory in WB.

Evaluated by Flow Cytometry in BALB/c splenocytes.

Flow Cytometry Analysis: 0.1 µg/mL of this antibody detected CD39 in BALB/c splenocytes.

Protein G Purified
Format: Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified antibody

primary antibodies

5F2, monoclonal

mouse

flow cytometry: suitable
western blot: suitable

IgG1κ

NP_033978

P55772

wet ice

~ 100 kDa
It has been published that CD39 shows molecular weights between ~70-100 kDa (Wang, T.F. et al., 1996).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1