Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27, rabbit monoclonal

32160702

NA.41

S6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that is known to be phosphorylated by both p70S6 Kinase as well as RSK as well as Ser240/244 which is thought to only be phosphorylated by p70S6K.

Broad species cross-reactivity predicted, due to sequence homology.
Recognizes ribosomal protein S6 only when phosphorylated on Ser235.

Synthetic peptide containing the sequence …RRL[pS]SL… in which pS corresponds to phospho-serine at residue 235 of human ribosomal protein S6. The immunizing sequence is identical in mouse, rat, chicken and Xenopus.
Epitope: Phosphorylated Ser235

Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27 is an antibody against phospho-Ribosomal Protein S6 (Ser235) for use in WB.
Western Blot Analysis:
A 1:500-1:2,000 dilution of this lot detected phosphorylated ribosomal protein S6 in lysates from serum stimulated 3T3 cells.
Research Sub Category
PI3K, Akt, & mTOR Signaling
Research Category
Signaling

Apoptosis & Cancer

Routinely evaluated by western blot on serum stimulated 3T3 cell lysate.

Cultured supernatant with 0.05% sodium azide.

Control
Serum stimulated 3T3 cell lysate.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

culture supernatant

primary antibodies

MC27, monoclonal

human, rat, mouse

Upstate^®

western blot: suitable

IgG

NM_001010.2

P62753

dry ice

human ... RPS6(6194)

~30 kDa

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1