Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8

32160702

NA.41

RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Ser-7 is phosphorylated before initiation of transcription at promoter regions.

This antibody recognizes RNA polymerase II subunit B1 at the CTD when phosphorylated at Ser5.

Epitope: Ser5
Ovalbumin-conjugated linear peptide corrresponding to human RNA polymerase subunit B1 CTD phosphorylated at Ser5.

Use Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 (Rat Monoclonal Antibody) validated in WB, ELISA, ChIP to detect RNA polymerase II subunit B1 (phospho-CTD Ser-5).
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.)
Research Sub Category
Transcription Factors

RNA Metabolism & Binding Proteins
Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Evaluated by Western Blot in γ-PPase untreated and treated NIH/3T3 cell lysates.

Western Blot Analysis: 1 µg/ml of this antibody detected RNA polymerase II CTD on 10 µg of γ-PPase untreated and treated NIH/3T3 cell lysates.

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
γ-protein phosphatase (γ-Ppase) untreated and treated NIH/3T3 cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

rat

purified immunoglobulin

primary antibodies

3E8, monoclonal

mouse

human (based on 100% sequence homology)

ChIP: suitable
ELISA: suitable
western blot: suitable

IgG2aκ

NP_000928

P24928

wet ice

~ 220 kDa

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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