Anti-MRGPRX4 Antibody, clone 3F4 ZooMAb^® Rabbit Monoclonal

rabbit

200

expressed in HEK 293 cells

unconjugated

purified antibody

primary antibodies

3F4, monoclonal
recombinant monoclonal

ZooMAb^® learn more

lyophilized

calculated mol wt ~ 36.46 kDa

rat, human

antibody small pack of 25 μL

recombinant expression
Learn more about Antibody Enhanced Validation

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

IgG

ambient

2-8℃

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Clone 3F4 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Mas-related G-protein coupled receptor member X4 (MRGPRX4). It targets an epitope within 16 amino acids from the C-terminal, cytoplasmic domain.

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal, cytoplasmic domain of human Mas-related G-protein coupled receptor member X4 (MRGPRX4).

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in rat spinal cord tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected MRGPRX4 in rat spinal cord tissue sections.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MRGPRX4 in lysates from Human Dorsal Root Ganglia, Human Spinal Cord, and U-87 and U251 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MRGPRX4 in SH-SY5Y cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-MRGPRX4, clone 3F4 ZooMAb^®, Cat. No. ZRB1078, is a recombinant Rabbit monoclonal antibody that specifically targets MRGPRX4 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Mas-related G-protein coupled receptor member X4 (UniProt: Q96LA9; also known as Sensory neuron-specific G-protein coupled receptor 5/6, MRGPRX4) is encoded by the MRGPRX4 (also known as MRGX4, SNSR5, SNSR6) gene (Gene ID: 117196) in human. MRGPRX4 is a multi-pass membrane glycoprotein with four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. It is shown to be localized in a subset of small dorsal root and trigeminal sensory neurons. It has been classified as an orphan receptor that is involved in the regulation of nociceptor function and/or development, including the sensation or modulation of pain. It is shown to be potently activated by enkephalins. Several unconjugated and conjugated bile acids are reported to act as ligands for MRGPRX4 and may contribute to cholestatic pruritus. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Meixiong, J., et al. (2019). Proc. Natl. Acad. Sci. USA. 116(21); 10525-10530; Yu, H., et al. (2019). eLife. 8; e48431).

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

11 - Combustible Solids

WGK 1