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Anti-p53 Antibody, clone 6O23, ZooMAb® Rabbit Monoclonal

产品编号:4082857
规格:recombinant, expressed in HEK 293 cells
包装规格:25 μL,4 X 25 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
408285725 μL2350
40828574 X 25 μL4400
产品性质
biological source【生物来源】
rabbit (recombinant)
recombinant【重组】
expressed in HEK 293 cells
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
6O23, recombinant monoclonal
product line【产品线】
ZooMAb® learn more
form【形式】
lyophilized
mol wt【分子量】
calculated mol wt 43.65 kDa
observed mol wt ~53 kDa
species reactivity
human
species reactivity (predicted by homology)
bovine, rhesus macaque
packaging【包装】
antibody small pack of 25 μL
greener alternative product characteristics【环保替代产品特性】
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation【增强验证】
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable (peptide)
isotype【同位素/亚型】
IgG
greener alternative category【环保替代产品分类】
Aligned
shipped in【运输】
ambient
storage temp.【储存温度】
2-8℃
基本信息
General description【一般描述】
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.
Specificity【特异性】
Clone 6O23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects p53 protein. It targets an epitope within 12 amino acids from the N-terminal region.
Immunogen【免疫原】
KLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal region of human p53.
Application【应用】
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected p53 in HEK293 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:10-100 dilution from a representative lot detected p53 in human ovarian cancer and human colon cancer tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p53 in A431 and HaCaT cell lines.

Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
Anti-p53, clone 6O23 ZooMAb , Cat. No. ZRB1175, is a highly specific recombinant rabbit monoclonal antibody that detects p53 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Physical form【外形】
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution【重悬】
300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Legal Information【法律信息】
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
产品说明
Target description【目标描述】
Cellular tumor antigen p53 (UniProt: P04637; also known as Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53) is encoded by the TP53 (also known as p53) gene (Gene ID: 7157) in human. p53, a well-conserved phosphoprotein, is one of the best-known tumor suppressors. Human p53 consists of 393 amino acids assembled into five structurally and functionally different domains. It acts as a sequence-specific nuclear transcription factor that binds to defined consensus sites within DNA as a tetramer and represses transcription of a set of genes involved in cell growth stimulation, while activating a different set of genes involved in cell cycle control. It causes growth arrest before either DNA replication thereby providing a window for DNA repair or elimination of cells with severely damaged DNA strands. In some cell types, however, p53 activation results in apoptosis as a means of eliminating severely damaged cells. In normal, non-activated cells, the p53 signaling network is not active. However, p53 signaling is activated in cells as a response to various signals. Carcinogen-induced DNA damage, abnormal proliferative signals, hypoxia, and loss of cell adhesion are some of the most common signals that activate p53. The final outcome of p53 activation depends on many factors and is mediated largely through the action of downstream effector genes transactivated by p53. In unstressed cells, p53 is latent and is maintained at low levels by targeted, ubiquitin-mediated degradation mediated by MDM2 and many other ubiquitin ligases. MDM2 functions as an E3 ligase to ubiquitinate p53 and force its export from the nucleus to the cytoplasm, where p53 is degraded by the proteasome. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Storage and Stability【储存及稳定性】
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 1
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