Anti-phospho-TrkB (Tyr816) Antibody, clone 2C21 ZooMAb^® Rabbit Monoclonal

rabbit (recombinant)

expressed in HEK 293 cells

unconjugated

purified antibody

primary antibodies

2C21, monoclonal
recombinant monoclonal

ZooMAb^® learn more

lyophilized

calculated mol wt 92 kDa
observed mol wt 110 kDa

rat, human

sheep, mouse, bovine

antibody small pack of 25 μL

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

recombinant expression
Learn more about Antibody Enhanced Validation

affinity binding assay: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable

IgG

Aligned

ambient

2-8℃

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Clone 2C21 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects TrkB phosphorylated on tyrosine 816.

KLH-conjugated linear peptide corresponding to 14 amino acids surrounding phosphotyrosine 816 from the C-terminal region of human TrkB.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-TrkB (Tyr816) in SH-SY5Y cells and in rat hippocampal cells treated with BDNF.

Peptide Inhibition Assay: Target band detection in lysate from HEK293 cells treated with BDNF (50 ng/mL, 30 min) was prevented by preblocking of a representative lot TrkB peptide phosphorylated on tyrosine 816, but not the corresponding non-phosphopeptide.

Affinity Binding Assay: A representative lot of this antibody bound TrkB peptide phosphorylated on tyrosine 816 with a KD of 3.8 x 10-9 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
This Anti-phospho-TrkB (Tyr816), clone 2C21 ZooMAb, Cat. No. ZRB1495, is a recombinant Rabbit monoclonal antibody that targets phospho-TrkB (Tyr816) and is tested in Affinity Binding Assay, Immunocytochemistry, Peptide Inhibition, and Western Blotting.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Pulmonary surfactant-associated protein C (UniProt: P11686; also known as SP-C, Pulmonary surfactant-associated proteolipid SPL(Val), SP5) is encoded by the SFTPC (also known as SFTP2) gene (Gene ID: 6440) in human. Pulmonary surfactant is a lipid-protein complex that promotes alveolar stability by lowering the surface tension at the air-fluid interface in the peripheral air spaces. Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. Four different surfactant-associated proteins have been described: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and two small hydrophobic proteins (SP-B and SP-C). SFTPC is synthesized with two pro-peptides (aa 1-23 and 59-197) that subsequently produce the SP-5 protein (aa 24-58). Mutations in SFTPC have been found in sporadic and familial idiopathic pulmonary fibrosis (IPF) in murine models. In human, mutations in SFTPC gene have been linked to Respiratory distress syndrome (RDS) in premature infants. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

11 - Combustible Solids

WGK 1