Anti-53BP1 Antibody, clone BP13 ZooMAb^® Mouse Monoclonal

200

mouse

expressed in HEK 293 cells

unconjugated

purified antibody

primary antibodies

BP13, monoclonal
recombinant monoclonal

ZooMAb^® learn more

lyophilized

calculated mol wt ~ 213.57 kDa
observed mol wt ~270 kDa

human

antibody small pack of 25 μL

recombinant expression
Learn more about Antibody Enhanced Validation

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

IgG1

ambient

2-8℃

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Clone BP13 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects human p53-binding protein 1.

Full-length recombinant human p53-binding protein 1.

Quality Control Testing

Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected TP53-binding protein 1 in HeLa cell nuclear extract.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected 53BP1 in human breast cancer tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected 53BP1 in HeLa cells treated with UV light.

Flow Cytometry Analysis: 1 μg from a representative lot detected 53BP1 in one million Hela cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-53BP1, clone BP13 ZooMAb^®, Cat. No. ZMS1044, is a recombinant Mouse monoclonal antibody that targets TP53-binding protein 1 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

TP53-binding protein 1 (UniProt: Q12888; also known as 53BP1, p53-binding protein 1, p53BP1) is encoded by the TP53BP1 gene (Gene ID: 7158) in human. 53BP1 is a homooligomeric double-strand break (DSB) repair protein that is involved in response to DNA damage and promotes non-homologous end joining (NHEJ)-mediated repair. It counteracts the function of the homologous recombination (HR) repair protein BRCA1. In response to DSB it undergoes phosphorylation by ATM, which promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites where it binds histone H2A monoubiquinated at lysine 15 (H2AK15Ub) and histone H4 dimethylated at lysine 20 (H4K20me2). Its Tudor-like region (aa 1484-1603) mediates its binding to H4K20m3e2. In the absence of DNA damage, it is phosphorylated at basal level, but following DSB and phosphorylation by ATM it interacts with PAX-interacting protein 1 (PAXIP1) and non-homologous end joining of dysfunctional telomeres. Three isoforms of 53BP1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Panier, S., and Boulton, S. (2014). Nat Rev Mol Cell Biol 15(1); 7-18).

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

11 - Combustible Solids

WGK 1