Anti-Influenza A Nucleoprotein Antibody, clone A1 ZooMAb^® Mouse Monoclonal

200

mouse

expressed in HEK 293 cells

unconjugated

purified antibody

primary antibodies

A1, recombinant monoclonal

recombinant, expressed in HEK 293 cells

ZooMAb^® learn more

lyophilized

calculated mol wt 56.21 kDa

influenza A virus

antibody small pack of 25 μL

recombinant expression
Learn more about Antibody Enhanced Validation

ELISA: suitable
immunocytochemistry: suitable

IgG2a

Unknown

P03466

ambient

2-8℃

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Clone A1 is a ZooMAb^® mouse recombinant monoclonal antibody that detects Nucleoprotein N1 of Influenza A virus.

Influenza A virus

Quality Control Testing

Evaluated by Immunocytochemistry in MDCK cells infected with Influenza A virus.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Influenza A Nucleoprotein in MDCK cells infected with Influenza A virus.

Tested applications

ELISA Analysis: 1.0 μg/mL from a representative lot detected recombinant Influenza A virus Nucleoprotein.

Affinity Binding Assay: A representative lot of this antibody bound recombinant Influenza A Nucleoprotein with a KD of 2.8 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Influenza A Nucleoprotein, clone A1 ZooMAb^®, Cat. No. ZMS1020, is a recombinant Mouse monoclonal antibody that detects Influenza A virus nucleoprotein and is tested for use in Affinity Binding Assay, ELISA, and Immunocytochemistry.

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Nucleoprotein (UniProt: P03466; also known as Nucleocapsid protein, Protein N) is encoded by the NP gene (Gene ID: 956531) in Influenza A virus. Influenza A viruses belong to the Orthomyxoviridae family and encapsidates the negative strand viral RNA that protects it from the action of nucleases. Its envelope membrane is reported to carry hemagglutinin, neuraminidase, and the ion channel protein M2. Inside the membrane there are layers of matrix protein 1 and ribonucleoprotein (RNP) complexes that contain viral RNA and binding proteins. RNP serves as template for transcription and replication and must be localized in the host cell nucleus to start an infectious cycle. However, due to its large size, RNP cannot diffuse through the nuclear pore complex and is transported through CRM-1-dependent nuclear export machinery. Viral nucleoprotein contains two nuclear localization signals (NLS) that are responsible for active RNP import into the nucleus. It has been reported that M1 interaction with RNP hides nucleoprotein′s NLS and soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. This dissociation of M1 from RNP unmasks nucleoprotein′s NLS, targeting the RNP to the nucleus. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

11 - Combustible Solids

WGK 1