Anti-Phospho-HDAC7-(Ser486) Antibody, clone 3O11 ZooMAb^® Rabbit Monoclonal

200

rabbit

expressed in HEK 293 cells

unconjugated

purified antibody

primary antibodies

3O11, recombinant monoclonal

recombinant, expressed in HEK 293 cells

ZooMAb^® learn more

lyophilized

calculated mol wt 102.93 kDa
observed mol wt ~42 kDa

using Protein A

human

mouse, monkey, bovine, rat

antibody small pack of 25 μL

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recombinant expression
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immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

IgG

Internal

NP_056216

Q8WUI4

peptide synthesis

ambient

2-8℃

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 3O11 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects HDAC7 phosphorylated on serine 486.

KLH-conjugated linear peptide corresponding to 10 amino acids surrounding phosphoserine 486 from the internal region of human Histone deacetylase 7 (HDAC7).

Quality Control Testing

Evaluated by Western Blotting with a construct containing MBP-tagged recombinant fragment of HDAC7 phosphorylated on serine 486.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected a construct containing MBP-tagged recombinant HDAC7 phosphorylated on serine 486 but did not react with non-phosphorylated construct (Phospho-HDAC7 construct: Courtesy of Dr. Jesse Rinehart, Yale University, School of Medicine)

Tested applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Phospho-HDAC7-(Ser486) in human prostate cancer tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Phospho-HDAC7-(Ser486) in HEK293 cells treated with epidermal growth factor (EGF; 50 ng/mL; 10 min.).

Peptide Inhibition Assay: Target band detection in a lysate from A431 was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Histone deacetylase 7 (UniProt: Q8WUI4; also known as EC:3.5.1.98, HD7, Histone deacetylase 7A, HD7a) is encoded by the HDAC7 (also known as HDAC7A) gene (Gene ID: 51564) in human. Histone acetylation and deacetylation play a major role in determining chromatin structure and function. These modifications are achieved by histone acetyltransferases (HATs) and histone deacetylases (HDACs). In the deacetylated state, specific basic amino acids in histones are positively charged and interact with DNA s negatively charged phosphate groups and with negatively charged patches on neighboring nucleosomes. This promotes chromatin condensation to form heterochromatin. On the other hand, acetylation neutralizes these positive charges and promoting the less condensed and more accessible chromatin conformation known as euchromatin. HDAC7 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). It shuttles between the nucleus and the cytoplasm and in the nucleus, it associates with distinct subnuclear dot-like structures. Its nuclear export signal is localized in amino acids 918-952. Its deacetylase activity maps to a C-terminal domain and is dependent on its interaction with other class I HDACs in the nucleus. HDAC7 can undergo phosphorylation by PKN1 and PKN2 that impairs its nuclear import. Its phosphorylation on serine 155 by MARK2, MARK3, and PRKD1 is shown to promote its interaction with 14-3-3 proteins and facilitate its export from the nucleus by binding to specific phosphoserine 486 on HDAC7. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

13 - Non Combustible Solids

WGK 1