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ER Export Inhibitor II, FLI-06-Calbiochem
产品编号: | 4083523 |
规格: | A cell-permeable compound that reversibly blocks cellular glycosylation and surface expression of Notch, APP, Klotho by blocking events prior to cargo assembly at the ER exit sites. |
包装规格: | 10 MG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
ER Export Inhibitor II, FLI-06-Calbiochem
Cyclohexyl 2,7,7-trimethyl-4-(4-nitrophenyl)-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate, Cyclohexyl 4-(4'-nitrophenyl)-2,7,7-trimethyl-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate, FLI06
基本信息
Empirical Formula【经验(实验)分子式】 | C25H30N2O5 |
Molecular weight | 438.52 |
General description【一般描述】 | A cell-permeable, non-toxic, dihydropyridine compound that prevents cellular maturation/glycosylation and surface expression of Notch, APP, Klotho (Effect. conc. = 10 µM in HEK293, HeLa, U2OS cultures) in a rapid and reversible manner by blocking events prior to cargo assembly at the endoplasmic reticulum exit sites (ERES) without affecting cytoskeleton, endocytosis, or other post-ERES events targeted by BFA, GCA, Exo1, A5, Retro-2, U18666A, or Vacuolin-1 (Cat. nos. 203729, 345862, 341220, 444805, 554715, 662015, & 673000, respectively). Notch signaling is shown to be effectively inhibited by treatment with either FLI-06 or the γ-Secretase inhibitior DAPT (Cat. nos. 565770 & 565784) in both C2C12 cultures (10 µM FLI-06) in vitro and in zebrafish embryos (50 µM FLI-06) in vivo. A cell-permeable, non-toxic, dihydropyrimidine compound that prevents cellular glycosylation and surface expression of Notch, APP, Klotho (Effect. conc. = 10 µM in HEK293, HeLa, U2OS cultures) in a rapid and reversible manner by blocking events prior to cargo assembly at the ER exit sites (ERES) without affecting other post-ERES events targeted by BFA, GCA, Exo1, A5, Retro-2, U18666A, or Vacuolin-1 (Cat. nos. 203729, 345862, 341220, 444805, 554715, 662015, & 673000, respectively). Notch signaling is shown to be effectively inhibited by treatment with either FLI-06 or the γ-Secretase inhibitior DAPT (Cat. nos. 565770 & 565784) in both C2C12 cultures (10 µM FLI-06) in vitro and in zebrafish embryos (50 µM FLI-06) in vivo. |
Biochem/physiol Actions【生化/生理作用】 | Cell permeable: yes Reversible: yes Primary Target ER |
Warning【警告】 | Toxicity: Standard Handling (A) |
Reconstitution【重悬】 | Following reconstitution, aliquot and freeze (-20°). Stock solutions are stable for up to 6 months at -20°C. Use only fresh DMSO for reconstitution. |
Other Notes【其他说明】 | Kramer, A., et al. 2013. Nat. Chem. Biol.9, 731. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
Assay【测定】 | ≥95% (HPLC) |
form【形式】 | powder |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze protect from light |
color【颜色】 | yellow |
solubility【溶解性】 | DMSO: 100 mg/mL |
storage temp.【储存温度】 | 2-8℃ |
packaging【包装】 | Packaged under inert gas |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 3 |