Luciferase Substrate, CycLuc1-Calbiochem

C₁₃H₁₁N₃O₂S₂

305.38

A synthetic cyclic alkylaminoluciferin that exhibits properties that are superior to those of D-luciferin. Binds to luciferase with higher affinity and and emits about 5.7-fold more light than aminoluciferin and 3.2-fold more light than D-luciferin. Its superior light output is maintained over a wide range (1-100 µM). Shown to readily cross the blood-brain barrier in mice and provides much improved noninvasive bioluminescence imaging (BLI) of the brain at lower dose when compared to D-luciferin. Yields about 10-fold higher bioluminescent signal than D-luciferin when Luc2 luciferase expressing 4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice. A rapid (4-5 min), long-lasting, and steady signal peak is observed following an i.v injection CycLuc1 in mice.

Please note that the molecular weight for this compound is batch-specific due to variable water content.
A synthetic cyclic alkylaminoluciferin that exhibits properties that are superior to those of D-luciferin. Binds to luciferase with higher affinity and and emits about 5.7-fold more light than aminoluciferin and 3.2-fold more light than D-luciferin. Its superior light output is maintained over a wide range (1-100 µM). Shown to readily cross the blood-brain barrier in mice and provides much improved noninvasive bioluminescence imaging (BLI) of the brain at lower dose when compared to D-luciferin. Yields about 10-fold higher bioluminescent signal than D-luciferin when Luc2 luciferase expressing 4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice. A rapid (4-5 min), long-lasting, and steady signal peak is observed following an i.v injection CycLuc1 in mice.

Cell permeable: yes
Primary Target
Luciferase

Toxicity: Standard Handling (A)

Evans, M., et al. 2014. Nat Methods.11, 393.
Reddy, G. R, et al. 2010. J. Am. Chem. Soc.132, 13586.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥94% (HPLC)

powder

Calbiochem^®

OK to freeze
protect from light

yellow

DMSO: 100 mg/mL

2-8℃

Packaged under inert gas

11 - Combustible Solids

WGK 3