Lipopolysaccharide, E. coli J5-Calbiochem

100

lyophilized

Calbiochem^®

OK to freeze

<2.5% Protein
5.0-10.0% 2-keto-3-deoxyoctonate (KDO)

water: 1 mg/mL

phosphate (PO₄^3-): 6.0-15.0%

ambient

2-8℃

5 mg in Plastic ampoule

Mutant of E. coli 0111:B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes. Highly purified preparation.
Mutant of E. coli O111: B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.

Primary Target
Inducible nitric oxide synthase
Product does not compete with ATP.
Reversible: no
Cell permeable: no

Toxicity: Harmful (C)

LPS will form a suspension in H₂O; slight warming and vortexing may be required to achieve complete dispersion in H₂O. Addition of 0.5% triethylamine will also help with dispersion; triethylamine is very basic and can be neutralized with Trix-HCl to avoid hydrolysis of the fatty acid chains.

Following reconstitution refrigerate (4°C). Stock solutions are stable for up to 1 year at 4°C.

Hattori, S., et al. 1995. Biochem. Mol. Biol. Int.35, 177.
Mayeux, P.R., et al. 1995. Biochem. Pharmacol.49, 115.
Norimatsu, M., et al. 1995. Infect. Immun.63, 1122.
Feinstein, D.L., et al. 1993. J. Neurochem.60, 1945.
Zhang, Y.H., et al. 1993. Infect. Immun.61, 5044.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

GHS06

Danger

H300

P264 - P270 - P301 + P310 - P405 - P501

Acute Tox. 2 Oral

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK 3