PDK1, His·Tag^®, Human, Recombinant, S. frugiperda

2.7.10

Full length, recombinant, human PDK1 fused to a His•Tag sequence and expressed in S. frugiperda insect cells. PDK1 is a serine/threonine protein kinase that is localized to the plasma membrane. PDK1 activates conventional protein kinase C (PKC) as well as PKC_ζ. PDK1 also phosphorylates Akt and plays a role in insulin signaling.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5.

In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% TRITON^™ X-100 Detergent, pH 7.5.

Following initial thaw, aliquot and freeze (-70°C).

Scott, M.T., et al. 2002. EMBO J.21, 6771.
Biondi, R.M., et al. 2000. EMBO J.19, 979.
Alessi, D.R., et al. 1997. Curr. Biol.7, 776.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

≥90% (SDS-PAGE)

100

liquid

≥75 units/mg protein

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−70℃

5 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

10 - Combustible liquids

WGK 2