Caspase-1 Substrate VI, Fluorogenic-CAS 219137-85-6-Calbiochem

C₃₃H₃₆F₃N₅O₁₀

719.66

Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke′s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively.

Primary Target
Fluorogenic caspase-1 substrate
Product does not compete with ATP.
Reversible: no
Cell permeable: no

Toxicity: Standard Handling (A)

Ac-Tyr-Val-Ala-Asp-AFC

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Jones, R.A., et al. 1998. Hepatology 27, 1632.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Walker, N.P., et al. 1994. Cell 78, 343.
Thornberry, N.A., et al. 1992. Nature 356, 768.

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100

98% (HPLC)

solid

Calbiochem^®

OK to freeze
desiccated
protect from light

white to off-white

DMSO: 5 mg/mL

λ_ex ~400  nm
λ_em ~505 nm

ambient

−20℃

5 mg in Plastic ampoule

11 - Combustible Solids

WGK 1