Cdk1 Inhibitor IV, RO-3306-Calbiochem

C₁₈H₁₃N₃OS₂

351.45

A cell-permeable quinolinyl thiazolinone compound that acts as a potent and ATP-competitive inhibitor of Cdk1 (K_i = 35 nM and 110 nM for Cdk1/B1 and Cdk1/A, respectively). It affects Cdk2/E, PKCδ, and SGK only at much higher concentrations (K_i = 340, 318, and 497 nM, respectively), and shows little effect against Cdk4/D and six other commonly studied kinases (K_i ≥ 2 µM). Short-term treatment for up to 20 hrs results in fully reversible G2/M cell cycle arrest, while prolonged treament (>48 hrs) results in apoptotic cell death in proliferating cancer cells, but not in nontumorigenic epithelial cell lines. Also available as a 10 mM solution in DMSO (Cat. No. 217721).

Cell permeable: yes
Target K_i: 35 nM and 110 nM for Cdk1/B1 and Cdk1/A, respectively
Reversible: no
Primary Target
Cdk1
Product competes with ATP.

Toxicity: Irritant (B)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Vassilev, L.T., et al. 2006. Proc. Natl. Acad. Sci. USA103, 10660.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

brown

DMSO: 10 mg/mL

ambient

2-8℃

5 mg in Glass bottle
Packaged under inert gas

11 - Combustible Solids

WGK 3