Propidium Iodide Solution-CAS 25535-16-4-Calbiochem

C₂₇H₃₄I₂N₄

668.39

MFCD00011921

Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.

Primary Target
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no
Cell permeable: no

Toxicity: Standard Handling (A)

50 µg/ml in PBS, pH 7.4.

Following initial use, aliquot and freeze (-20°C).

Belloc, F., et al. 1994. Cytometry17, 59.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

liquid

Calbiochem^®

OK to freeze
protect from light

sample type intact cells

λ_ex 536 nm
λ_em 617 nm

ambient

2-8℃

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

XJMOSONTPMZWPB-UHFFFAOYSA-M

5 ml in Plastic ampoule

12 - Non Combustible Liquids

WGK 1