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Nitrocefin-CAS 41906-86-9-Calbiochem
| 产品编号： | 4084484 |
| 规格： | A chromogenic β-lactamase substrate that undergoes distinctive color change from yellow as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase. |
| CAS NO： | 41906-86-9 |
| 包装规格： | 5 MG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
41906-86-9
Nitrocefin-CAS 41906-86-9-Calbiochem
3-(2,4-Dinitrostyryl)-(6R, 7R)-7-(2-thienylacetamido)-ceph-3-em-4-carboxylic Acid, E-isomer
基本信息
| Empirical Formula【经验(实验)分子式】 | C21H16N4O8S2 |
| Molecular weight | 516.50 |
| General description【一般描述】 | A chromogenic β-lactamase substrate that undergoes distinctive color change from yellow (λmax = 390 nm at pH 7.0) to red (λmax = 486 nm at pH 7.0) as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase. Nitrocefin is sensitive to hydrolysis by all known lactamases produced by Gram-positive and Gram-negative bacteria. Also useful for the detection of β-lactamase patterns from bacterial cell extracts by isoelectric focusing. Has been used in competitive inhibition studies in developmental work on β-lactamase-resistant antibiotics. |
| Warning【警告】 | Toxicity: Flammable (J) |
| Preparation Note【制备说明】 | Preparing a Nitrocefin (500 µg/ml) Solution • Dissolve 1 mg Nitrocefin in 100 µl dimethylsulfoxide (DMSO) and vortex. • Add 1.9 ml phosphate buffer (100 mM, pH 7) to produce 2 ml total volume. • This yields a working Nitrocefin solution of 500 µg/ml (~1 mM), which is suitable for most applications. • Nitrocefin, particularly in solution, is very sensitive to light. |
| Reconstitution【重悬】 | Following reconstitution, aliquot and freeze (-20°C). Stock solution may be stored at -20°C for up to 2 weeks. |
| Other Notes【其他说明】 | Guay, R., et al. 1980. IRCS Med. Science 8, 209. King, A., et al. 1980. Antimicrob. Agents Chemother. 17, 165. Matthew, M., et al. 1975. J. Gen. Microbiol. 88, 169. O’Callaghan, C.H., et al. 1972. Antimicrob. Agents Chemother. 1, 283. Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 200 |
| Assay【测定】 | ≥95% (UV) |
| form【形式】 | solid |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze protect from light |
| color【颜色】 | orange-yellow |
| shipped in【运输】 | ambient |
| storage temp.【储存温度】 | −20℃ |
| InChI | 1S/C21H16N4O8S2/c26-16(9-14-2-1-7-34-14)22-17-19(27)23-18(21(28)29)12(10-35-20(17)23)4-3-11-5-6-13(24(30)31)8-15(11)25(32)33/h1-8,17,20H,9-10H2,(H,22,26)(H,28,29)/b4-3+/t17-,20-/m1/s1 |
| InChI key | LHNIIDJCEODSHA-OQRUQETBSA-N |
| packaging【包装】 | 5 mg in Glass bottle Packaged under inert gas |
安全信息
| Pictograms【象形图】 | GHS05,GHS07 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H315 - H317 - H318 |
| Precautionary Statements | P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338 |
| Hazard Classifications【危险分类】 | Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1 |
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 3 |
