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Anti-Giα2 Antibody, clone L5
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| Specificity【特异性】 | Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB. This antibody recognizes Giα-2. |
| Immunogen【免疫原】 | Recombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243). |
| Application【应用】 | Research Sub Category GPCR, cAMP/cGMP & Calcium Signaling This Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2. Research Category Signaling Optimal working dilutions must be determined by end user. Immunohistochemistry(paraffin): Representative testing from a previous lot. Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes |
| Quality【质量】 | Routinely evaluated by Western Blot on Huvec lysates. Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates. |
| Linkage【联系】 | Replaces: MAB3077 |
| Physical form【外形】 | Purified Format: Purified Purified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide. |
| Analysis Note【分析说明】 | Control Huvec cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | L5, monoclonal |
| species reactivity | mouse (69%), human (62%), guinea pig (43%), bovine (100%), rat (69%) |
| technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
| isotype【同位素/亚型】 | IgG2b |
| NCBI accession no.【NCBI登记号】 | NM_002070.2 |
| UniProt accession no.【UniProt登记号】 | P04899 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... GNAI2(2771) |
产品说明
| Target description【目标描述】 | Labels a single protein band of 39-42 kDa in bovine and rat membranes. |
| Storage and Stability【储存及稳定性】 | Maintain at 2-8°C for 1 year from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 2 |
