Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306

32160702

NA.41

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

This antibody recognizes monomethylated Lys9 at and around the N-terminal of Histone H3.

KLH conjugated linear peptide corresponding to monomethylated Lys9 at the N-terminal region of Histone H3.
Epitope: monomethylated H3 Lys9

Dot Blot Analysis: AbSurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 05-1248, Anti-monomethyl H3 (Lys9) at 2ug/mL (1:500) dilution. Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system.

Peptide Inhibition: Representative lot data. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in western blot analysis of HeLa acid extracts and various other modified histone peptides.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Use Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306 (Mouse Monoclonal Antibody) validated in WB, PIA to detect monomethyl Histone H3 (Lys9) also known as H3K9me1.

Evaluated by Western Blot on recombinant Histone H3 and HeLa acid extract.
Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3 in 500 ng of recombinant Histone H3 and 10 µg of HeLa acid extract.

Format: Purified
Purified mouse monoclonal IgG2a in PBS with 0.05% sodium azide.
Protein G Purified

Control
Recombinant Histone H3 and HeLa acid extract.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

CMA306, monoclonal

human

inhibition assay: suitable (peptide)
western blot: suitable

IgG2a

NP_003484

O43463

wet ice

~17 kDa

Stable at 2-8°C for 1 year from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2