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Topo I (197-651) (core domain) human
| 产品编号： | 4084964 |
| 规格： | recombinant, expressed in insect cells, ≥80% (SDS-PAGE) |
| 包装规格： | 5 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Topo I (197-651) (core domain) human
TOPI
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in insect cells |
| Assay【测定】 | ≥80% (SDS-PAGE) |
| form【形式】 | frozen liquid |
| mol wt【分子量】 | ~56 kDa |
| packaging【包装】 | pkg of 5 μg |
| storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
| concentration【浓度】 | 500 μg/mL |
| color【颜色】 | clear colorless |
| NCBI accession no.【NCBI登记号】 | NM_003286 |
| UniProt accession no.【UniProt登记号】 | P11387 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... TOP1(7150) |
基本信息
| Biochem/physiol Actions【生化/生理作用】 | Human DNA topoisomerase I is the best studied of the DNA topoisomerase family. It catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. The core domain spanning from amino acids 215 to 636 is highly conserved and retains DNA binding activity. Topo I has been found to nick the DNA with a preference of 5′-(A/T)(G/C)(A/T)T-3′. Camptothecin and its analogs have been tested as potent anticancer compounds by stabilizing topo I-DNA complex, thereby inhibiting both DNA and RNA synthesis. |
| Physical form【外形】 | Clear and colorless frozen liquid solution |
| Preparation Note【制备说明】 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
