Anti-IGF-IR Antibody, β-subunit, clone 1-2

32160702

NA.41

IGF-I and -II signal through the IGF-I Receptor, which is homologous to the Insulin Receptor. The high-affinity IGF-II Receptor does not play a direct role in signaling, but regulates the concentration of free IGF-II. The IGFs are involved in skeletal growth, and are essential for prevention of apoptosis. Serum levels of free IGFs are kept low by the action of IGF binding proteins (IGFBPs), which sequester the IGFs. Overexpression of IGFBPs may induce apoptosis, presumably by reduction of free IGF; IGFBP levels are also altered in some cancers. The IGF-I Receptor is not as mitogenic as some other growth factor receptors, but its ability to activate the PI3 Kinase pathway, through the Insulin Receptor Substrate (IRS) proteins, is critical for mediating cell survival.

Recognizes the β-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 1323-1337 of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the α- and β-subunits, which are disulfide-linked in a β-α-α-β configuration in the mature receptor. The α-subunit is completely extracellular, while the β-subunit spans membrane.

Epitope: Between a.a. 1323-1337 of IGF-1R
A 15-mer peptide corresponding to a.a. 1323-1337 (Tyr-RKNERALPLPQSSTC) from the C-terminal of β-subunit of human IGF-1R.

Immunohistochemistry(paraffin): Representative images from a previous lot.
Optimal Staining of IGF Monoclonal Antibody: Placenta

Western Blot: A previous lot of this antibody was used at 1-2 µg/mL for 2 hours at room temperature.

Optimal working dilutions must be determined by end user.

Research Sub Category
Insulin/Energy Signaling
Anti-IGF-IR Antibody, β-subunit, clone 1-2 is an antibody against IGF-IR for use in WB, IH(P).
Research Category
Signaling

Apoptosis & Cancer

Routinely evaluated by Western Blot on C2C12 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected IGF-1 Receptor beta on 10 μg of C2C12 lysates.

Replaces: MAB1123

Purified mouse monoclonal IgG2b in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Affinity purified

Control
C2C12 Cell Lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

affinity isolated antibody

primary antibodies

1-2, monoclonal

affinity chromatography

mouse, rat

human

immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG2b

NM_000875.3

P08069

wet ice

human ... IGF1R(3480)

Recognizes the beta-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R; ~140 kDa)

Stable at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2