Anti-mTOR Antibody, clone 21A12.2

32160702

NA.41

mTOR (Mammalian Target of Rapamycin, FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 consists of at least mTOR, Raptor, and GL. mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The other mTOR complex, mTORC2, is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. TORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473. TORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

This antibody only detects mTOR.
Other species to be tested.

Full-length human mTOR

Confocal Immunofluorescent Immunocytochemistry:
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
Detect mTOR using this Anti-mTOR Antibody, clone 21A12.2 validated for use in WB & IC.

Evaluated by Western Blotting on HEK293 cell lysates.

Western Blotting Analysis: 1:1,000 - 2,000 dilution of this antibody was used to detect mTOR in HEK293 cell lysate.

Format: Purified
Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl, with 0.05% sodium azide.
Protein G Purified

Control
HEK293 cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified antibody

primary antibodies

21A12.2, monoclonal

human

immunocytochemistry: suitable
western blot: suitable

IgG1κ

NP_004949.1

Q6R327

wet ice

~289 kDa

Stable for 1 year at 2-8ºC from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1