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Anti-Sin1 Antibody, clone 1C7.2
产品别名
Anti-Sin1 Antibody, clone 1C7.2
MEKK2-interacting protein 1, Mitogen-activated protein kinase 2-associated protein 1, SAPK-interacting protein 1, Stress-activated map kinase-interacting protein 1, TORC2 subunit MAPKAP1, mitogen-activated protein kinase associated protein 1, ras inhibit
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Sin1 (stress-activated protein kinase (SAPK)-interacting protein 1, MAPKAP1) is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase along with Sin1, GL (mLST8), Protor1, Protor2, and Rictor. The complex that is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Sin1 is also known to directly interact without proteins that include Ras, SPAKs, and JNK. Sin1 is known to have multiple alternative splicing isoforms. Full-length Sin1 is a 522aa protein (59kDa). Sin1 (Isoform 5) (36 kDa) has exon 6 spliced to an alternative exon 7a that results in the loss of both the RBD and PHL domains. Sin1Isoform 2) (55 kDa) lacks exon 7 and lacks the RBD. Sin1 (Isoform 3) (53 kDa) lacks exon 8 lacks the PHL domain. Sin1 (Isoform 4) (37 kDa) lacks exon 1. |
| Specificity【特异性】 | Other species have not been tested. Detects Sin-1 and its isoforms. |
| Immunogen【免疫原】 | Full-length Human Sin1 GST-fusion protein. |
| Application【应用】 | Detect Sin1 using this Anti-Sin1 Antibody, clone 1C7.2 validated for use in WB, IP, IH(P) & IF. |
| Quality【质量】 | Western Blot Analysis: This lot detected Sin1 at 1:1,000 dilution in A431 cell lysate resolved via SDS-PAGE and transferred to PVDF. |
| Physical form【外形】 | Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% NaN3. Protein G Purified Format: Purified |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 1C7.2, monoclonal |
| species reactivity | rat, human, mouse |
| technique(s) | immunofluorescence: suitable immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG2aκ |
| NCBI accession no.【NCBI登记号】 | NP_001006618 |
| UniProt accession no.【UniProt登记号】 | Q9BPZ7 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | Sin1 is 59 kDa Isoforms are 36, 37, 41, 53, 54, and 55 kDa |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
