Purmorphamine-CAS 483367-10-8-Calbiochem

C₃₁H₃₂N₆O₂

520.62

A cell-permeable 2,6,9-trisubstituted purine compound that induces osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2 (EC₅₀ = 1 µM) and lineage-committed preosteoblasts MC3T3-E1. Its effect can be synergized with that of BMP-4 (bone morphogenetic protein-4) and has been shown to induce transdifferentiation even in preadipocytes and myoblasts. Reported to induce osteogenesis by activation of the Hedgehog signaling pathway. A 50 mM (5 mg/192 µl) solution of Purmorphamine (Cat. No. 540223) in DMSO is also available.

Please note that the molecular weight for this compound is batch-specific due to variable water content.
A cell-permeable activator of Hedgehog signaling that induces osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2 (EC₅₀ = 1 µM).
A cell-permeable purine compound that induces osteoblast differentiation in C3H10T1/2 multipotent mesenchymal progenitor cells (EC₅₀ = 1 µM) and in MC3T3-E1 lineage-committed preosteoblasts. Also shown to induce transdifferentiation in pre-adipocytes and myoblasts. Its effects are reported to be synergistic with bone morphogenetic protein-4 (BMP-4). Reported to induce osteogenesis by activation of the Hedgehog signaling pathway.

Cell permeable: yes
Primary Target
Activation of the Hedgehog signaling pathway
Product does not compete with ATP.
EC50 = 1 µM for inducing osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2

Toxicity: Harmful (C)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Wu, X., et al. 2004. Chem. Biol.11, 1229.
Wu, X., et al. 2002. J. Am. Chem. Soc.124, 14520.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

≥98% (HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

off-white

DMSO: 200 mg/mL

ambient

2-8℃

5 mg in Plastic ampoule
20 mg in Glass bottle

GHS08

Warning

H341

P201 - P202 - P280 - P308 + P313 - P405 - P501

Muta. 2

11 - Combustible Solids

WGK 3