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粒细胞巨噬细胞集落刺激因子 人
Granulocyte-Macrophage Colony-Stimulating Factor human
| 产品编号： | 4085850 |
| 规格： | GM-CSF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture |
| 包装规格： | 10 μG，5 X 10 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
粒细胞巨噬细胞集落刺激因子 人
Granulocyte-Macrophage Colony-Stimulating Factor human
GM-CSF human
GM-CSF 人
基本信息
| MDL number | MFCD00166611 |
| NACRES | NA.77 |
| Quality【质量】 | endotoxin tested |
| General description【一般描述】 | Granulocyte-Macrophage Colony-Stimulating Factor is mapped to human chromosome 5q21-q32. |
| Application【应用】 | Granulocyte-Macrophage Colony-Stimulating Factor human has been used as a colony-stimulating cytokine in human acute myeloid leukemia cell line (OCI-M2) to test its effect on 5-azacitidin (AZA)-mediated DNA demethylation of upstream regulatory element (URE). |
| Biochem/physiol Actions【生化/生理作用】 | Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is an immune modulator and proinflammatory cytokine. It binds to the heteromeric cell-surface receptors present in the immune cells like granulocytes, endothelial cells and macrophages. GM-CSF is implicated in inflammation associated disorder of the lung and renal system. 粒细胞-巨噬细胞集落刺激因子 (GM-CSF) 是粒细胞、巨噬细胞和嗜酸性粒细胞系的生长和分化因子。GM-CSF 在极低浓度下刺激多能祖细胞集落形成，是造血祖细胞直至成熟的所有分裂中必不可少的存活和增殖因子。它也刺激一些上皮细胞和破骨细胞的生长。GM-CSF 由多种细胞类型(单核细胞、内皮细胞、T 细胞、成纤维细胞、有丝分裂原刺激的 B 细胞和 LPS 刺激的巨噬细胞)产生。GM-CSF 是一种单链糖蛋白，含有 128 个氨基酸，具有保守的二硫键。人和鼠 GM-CSF 的序列同源性约为 54%，生物活性无交叉反应。 |
| Caution【注意】 | Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity. |
| Physical form【外形】 | Lyophilized from a 0.2 μm filtered solution of 1x PBS. |
| Preparation Note【制备说明】 | HumanKine GM-CSF is expressed as a 15-36 kDa glycosylated monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity. |
| Analysis Note【分析说明】 | The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line). |
| Legal Information【法律信息】 | HumanKine is a registered trademark of Proteintech Group, Inc. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in HEK 293 cells |
| Assay【测定】 | ≥95% (SDS-PAGE) |
| form【形式】 | lyophilized powder |
| potency【效能】 | ≤5 ng/mL EC50 |
| mol wt【分子量】 | dimer 15-36 kDa (glycosylated) |
| packaging【包装】 | pkg of 5X10 μg pkg of 10 μg |
| storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
| technique(s) | cell culture | mammalian: suitable |
| impurities【杂质】 | ≤1 EU/mg |
| UniProt accession no.【UniProt登记号】 | P04141 |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... CSF2(1437) |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
