Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor^® 488 Conjugate

32160702

Insulin-like growth factor I (UniProt P05019; also known as IGF-I, Somatomedin-C) is encoded by the IGF1 (also known as IGFI, MGF) gene (Gene ID 3479) in human. Human insulin-like growth factor-I (IGF-I) is a single chain 70 amino acid polypeptide that shares structural homology with insulin and human IGF-II. IGF-I plays a critical role in growth, differentiation and development via autocrine, paracrine and endocrine mechanisms. It is a mitogen and also has effects on cell migration and differentiation. IGF-I is a regulator of metabolism and is also implicated in the pathophysiology of cancer, diabetes and obesity. Upon binding the tetrameric (alpha:beta)2 IGF-I receptor, the receptor alpha chains induce the tyrosine autophosphorylation of the beta chains, which in turn trigger cell type-specific downstream signaling cascade of events.

IGF-I (Russel, W.E., (1984). Proc. Natl. Acad. Sci. U. S. A. 81(8):2389-2392). 40% cross reactivity to IGF-II (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522).

Highly purified human plasma insulin-like growth factor I (IGF-I; Somatomedin C; Sm-C) conjugated to mouse albumin (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522). Action of somatomedins on cell growth: Effect of selective neutralisation of somatomedin C (IGF-I) with a monoclonal antibody. In: Raiti, S., Tolman, and R.A. (eds.), Human Growth Hormone. pp. 585–599. Plenum Publishing Corp., New York, NY).

Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor^® 488 Conjugate is an antibody against IGF-I for use in Immunocytochemistry.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IGF-I in HeLa and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-172) is shown to be suitable also for Western blotting, immunohistochemistry, immunoprecipitation, and neutralization applications.

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected IGF-I in A431 cells.

Purified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1 % sodium azide.
Protein G Purified

Concentration: Please refer to lot specific datasheet.

ALEXA FLUOR is a registered trademark of Life Technologies

100

mouse

ALEXA FLUOR^® 488

purified antibody

primary antibodies

Sm1.2, monoclonal

human, fish, rat, chicken, mouse

immunocytochemistry: suitable

IgG1κ

NP_000609

P05019

wet ice

human ... IGF1(3479)

~17 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2