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Anti-c-Jun Antibody, clone 6E4.4
| 产品编号： | 4087233 |
| 规格： | clone 6E4.4, from mouse |
| 包装规格： | antibody small pack of 25 μg |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-c-Jun Antibody, clone 6E4.4
JNK1 alpha protein kinase, JNK1 beta protein kinase, JUN N-terminal kinase, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated pro
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun. |
| Specificity【特异性】 | This antibody detects c-Jun, regardless of phosphorylation state. |
| Immunogen【免疫原】 | GST-tagged recombinant protein corresponding to c-Jun. |
| Application【应用】 | Western Blot Analysis (SNAP ID Technique): 5 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate using the SNAP ID Tecnique). Immunocytochemistry Analysis: Confocal fluorescent analysis of HeLa, C2C12, and A431 cells using anti-c-Jun, clone 6E4.4 mouse monoclonal antibody (see page 2). Research Sub Category Transcription Factors Anti-c-Jun Antibody, clone 6E4.4 is a Mouse Monoclonal Antibody for detection of c-Jun also known as JNK1 alpha protein kinase, JNK1 beta protein kinase & has been validated in WB, ICC. Research Category Epigenetics & Nuclear Function |
| Quality【质量】 | Evaluated by Western Blot in NIH/3T3 cell lysate. Western Blot Analysis: 0.5-2 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate. |
| Linkage【联系】 | Replaces: 04-210 |
| Physical form【外形】 | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G Purified |
| Analysis Note【分析说明】 | Control NIH/3T3 cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 6E4.4, monoclonal |
| species reactivity | mouse, human, rat |
| packaging【包装】 | antibody small pack of 25 μg |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG1κ |
| NCBI accession no.【NCBI登记号】 | NP_002219.1 |
| UniProt accession no.【UniProt登记号】 | P45983 |
| shipped in【运输】 | ambient |
产品说明
| Target description【目标描述】 | ~36-43 kDa Multiple bands may be visible, indicating various phosphorylation states. |
| Storage and Stability【储存及稳定性】 | Stable at 2-8°C for 1 year from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
