Anti-NPTII antibody produced in rabbit

MFCD01633903

NA.41

Neomycin phosphotransferase II (NPTII) gene also known as the kanamycin-resistance marker gene, encoding for NPTII (30 kDa) an aminoglycoside 3′ -phosphotransferase II (APHII), was isolated from the E. coli transposon Tn5.

The antibody recognizes bacterial NPTII expressed in transgenic tobacco plants.

synthetic peptide corresponding to amino acids 133-150 located at the mid-region of E. coli NPTII, conjugated to KLH.

Anti-NPTII antibody has been used in immunoblotting.

The Neomycin phosphotransferase II (NPTII) gene confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin in bacteria and plant cells and to G418 (Gibco Geneticin reagent) sulfate in mammalian cells. NPTII phosphorylates and inactivates kanamycin, preventing kanamycin from binding to the 30S ribosomal subunit to inhibit protein synthesis, thus rendering cells resistant to the antibiotic. NPTII serves as a marker for the selection of genetically engineered crops.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen 30 kDa

plant

recombinant expression
Learn more about Antibody Enhanced Validation

western blot: 2-4 μg/mL using extract (cytosolic fraction) from the leaves of transgenic Nicotiana tabacum expressing E. coli NPTII

dry ice

−20℃

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