Anti-PABP-2 Antibody

32160702

NA.41

PABPN1, also known as Polyadenylate-binding protein 2, Poly(A)-binding protein II (PABP-2), Poly(A)-binding protein 2, PABII, Nuclear poly(A)-binding protein 1,or Polyadenylate-binding nuclear protein 1, and encoded by the gene PABPN1/PAB2/PABP2, is a RNA binding protein involved in the 3′-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tails. PABPN1 stimulates poly(A) polymerase (PAPOLA) tail elongation and controls the poly(A) tail length. PABPN1is also important in nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA and appears to also protect the poly(A) tail from degradation. PABPN1 is localized to the nucleus and cytoplasm. PABPN1 is found in cytoplasmic mRNP granules containing untranslated mRNAs and shuttles between the nucleus and the cytoplasm but is predominantly found in the nucleus.

Recombinant protein corresponding to bovine PABP-2.

This Anti-PABP-2 antibody is validated for use in western blotting, IP & ICC for the detection of PABP-2.
Research Category
Epigenetics & Nuclear Function
Immunoprecipitation Analysis: A representative lot immunoprecipitated PABP-2 in mouse limb tissue lysate.
Immunocytochemistry Analysis: A representative lot detected PABP-2 in mouse limb cells.

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PABP-2 in 10 µg of A431 cell lysate.

Unpurified
Rabbit polyclonal in buffer containing serum with 0.05% sodium azide.

rabbit

100

serum

primary antibodies

polyclonal

rat, mouse, human

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NP_776994

Q28165

wet ice

human ... PABPN1(8106)

~ 50 kDa observed. The calculated molecular weight is 34 kDa, however PABP-2 has been shown as a ~50 kDa band in western blots (Nemeth A. K, et al. (1995). Nucleic Acids Res. 23(20):4034-41).

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1