Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11)

100

mouse

purified antibody

primary antibodies

II-4C11, monoclonal

liquid

≤0.1% sodium azide as preservative

rat, rabbit, human, bovine

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG1

wet ice

−20℃

100 μg in Plastic Bag(s)
Please refer to vial label for lot-specific concentration.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-X-63-Ag8-LUI mouse myeloma cells. Recognizes the ~130 kDa type II collagen protein.
Recognizes the ~130 kDa type II collagen protein. May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected.
This Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11) is validated for use in Frozen Sections for the detection of Collagen Type II (Ab-1).

Human
purified collagen type II

Frozen Sections (1-10 µg/ml)

Immunoblotting (1-5 µg/ml, see application references)

Paraffin Sections (1-10 µg/ml, see application references)

Toxicity: Standard Handling (A)

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Following initial thaw, aliquot and freeze (-20°C).

May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected. This antibody may also react with other classes of collagen at a concentration higher than 1 µg/ml. In immunoblotting, a major band of ~130 kDa is obtained. Antibody should be titrated for optimal results in individual systems.
Kumagai, J. et al. 1994 J. Anat.185, 278.
Kivrikko, K,I. 1993 Ann. Med.25, 113.
Sandell, L.J., et al. 1991 J. Cell. Biol.114, 1307.
Ryan, M.C., and Sandell, L.J. 1990 J. Biol. Chem.265, 10334.
Mayne, R., and Burgeson, R.E. 1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

12 - Non Combustible Liquids

WGK 1