Anti-Fibronectin (Ab-3) Mouse mAb (FBN11)

100

mouse

purified antibody

primary antibodies

FBN11, monoclonal

liquid

≤0.1% sodium azide as preservative

human, mouse, rat

Calbiochem^®

do not freeze

IgG1

wet ice

2-8℃

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections).
Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue.
Protein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin.

recombinant human fibronectin

Frozen Sections (0.5-1 µg/ml)

Immunoblotting (0.5-1 µg/ml)

Immunofluorescence (see comments)

Immunoprecipitation (2 µg/mg protein lysate)

Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)

Toxicity: Standard Handling (A)

Positive Control
SW 156 cells or normal kidney tissue

Exhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Clark, R.A. 2001. Ann. N.Y. Acad. Sci.936, 355.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.

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11 - Combustible Solids

WGK 1