PhosphoDetect Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

liquid

≤0.1% sodium azide as preservative

rat, mouse, human

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG

wet ice

−20℃

10 tests in Plastic ampoule

Recognizes c-Raf phosphorylated at Tyr³⁴⁰ and Tyr³⁴¹ in HEK293 cells overexpressing c-Raf.
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~74 KDa c-Raf protein phosphorylated on Tyr^340/341.
This PhosphoDetect Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb is validated for use in WB, ICC for the detection of c-Raf (pTyr³⁴⁰, Tyr³⁴¹).
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1)
Human

Immunoblotting (1:1000)

Immunocytochemistry (see comments)

Toxicity: Standard Handling (A)

In phosphate buffer (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3 (+/- 0.1).

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
HEK293 cells over expressing c-Raf, phosphorylated in vitro by Src.

The immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Yan, F., and Polk, D.B. 2001. Cancer Res.61, 963.
Yip-Schneider, M.T., et al. 2000. Biochem. J.351, 151.
Mason, C.S. et al. 1999. EMBO J.18, 2137.
Bonner, T.I. et al. 1985. Mol. Cell. Biol.5, 1400.
Moelling, K. et al. 1984. Nature312, 558.
Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA80, 4218.

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12 - Non Combustible Liquids

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