Anti-Calreticulin (405-417) Rabbit pAb

rabbit

100

purified antibody

primary antibodies

polyclonal

liquid

≤0.1% sodium azide as preservative

porcine, human, canine, bovine, monkey, mouse, hamster, rat

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG

wet ice

−20℃

50 μL in Plastic ampoule

Protein A purified rabbit polyclonal antibody. Recognizes the ~63 kDa calreticulin protein.
Recognizes the ~63 kDa calreticulin protein in heat-shocked HeLa cells.
Anti-Calreticulin (405-417), rabbit polyclonal, recognizes the ~63 kDa calreticulin in heat-shocked HeLa cells. It is validated for WB, ICC, IHC, and IP.

Human
a synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH

Immunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent)

Immunocytochemistry (see comments)

Immunoprecipitation (see comments)

Immunohistochemistry (see comments)

Toxicity: Standard Handling (A)

In PBS, 50% glycerol, pH 7.2.

Following initial thaw, aliquot and freeze (-20°C).

Does not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Janicki, S., et al. 1997. J. Cell Biol. 139, 485.
Wada, I., et al. 1997. EMBO J. 16, 5420.
Nauseef, W.F., et al. 1995. J. Biol. Chem.270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.

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10 - Combustible liquids

WGK 1