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Anti-Atg4D Antibody
| 产品编号： | 4087686 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Atg4D Antibody
Cysteine protease ATG4D, AUT-like 4 cysteine endopeptidase, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Atg4D (autophagy-related protein 4D) is a member of the Atg4 family of C54 endopeptidases that are required for the synthesis of autophagosomes. Atg4D is found to be cleaved by caspase-3 into a more active form where it can interact with the autophagosome marker Atg8. Atg4D is also known to be associated with mitochondrial dysfunction and the induction of apoptosis. |
| Specificity【特异性】 | This antibody recognizes Atg4D at the N-terminus. |
| Immunogen【免疫原】 | Epitope: N-terminus KLH-conjugated linear peptide corresponding to the N-terminus of human Atg4D. |
| Application【应用】 | Anti-Atg4D Antibody is an antibody against Atg4D for use in WB, IH(P). Research Sub Category Apoptosis - Additional Research Category Apoptosis & Cancer Immunohistochemistry (Paraffin) Analysis: A 1:200 dilution of a previous lot detected Atg4D in human testicular cancer tissue. Western Blot Analysis: 1 µg/mL from a representative lot detected Atg4D on 10 µg of human fetal skeletal muscle tissue, NIH/3T3, and L6 cell lysates. |
| Quality【质量】 | Evaluated by Western Blot in A431 cell lysate. Western Blot Analysis: 0.05 µg/mL of this antibody detected Atg4D in 10 µg of A431 cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control A431 cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | mouse, rat, human |
| species reactivity (predicted by homology) | rhesus macaque (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_116274 |
| UniProt accession no.【UniProt登记号】 | Q86TL0 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... ATG4D(84971) |
产品说明
| Target description【目标描述】 | ~56 kDa observed. The calculated molecular weight is 53 kDa; however, Atg4D has been shown as a ~55 kDa band in western blots (Li, M., et al. (2011). J Biol Chem. 286(9):7327-7338.) |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
