Anti-CUX1 Antibody, a.a. 861

32160702

NA.41

Homeobox protein cut-like 1 (CUX1) is also known as CCAAT displacement protein (CDP) and Homeobox protein cux-1. CUX1 is thought to repress expression of developmentally regulated genes involved in mammalian development, likely through the prevention of CCAAT factor binding at promoters. CUX1 also represses T-cell receptor (TCR) beta enhancer function by binding to matrix attachment region beta (MARbeta), and is a component of the nf-munr repressor, which binds to matrix attachment regions (MARs) of the immunoglobulin heavy chain enhancer.

This antibody recognizes the region of amino acids beginning at 861 of CUX1. This antibody is expected to cross react with all known isoforms of CUX1.
Other homologies: Mouse (95% sequence homology).

Epitope: Region of amino acids beginning at 861
GST-tagged recombinant protein corresponding to a region of amino acids beginning at 861 of human CUX1.

Research Sub Category
Transcription Factors
Immunoprecipitation Analysis: 1.25 µg from a representative lot immunoprecipitated CUX1 in 500 µg of HeLa nuclear extract.

Alexa Fluor^® is a registered trademark of Life Technologies.
Detect Cux1 using this rabbit polyclonal antibody, Anti-CUX1 Antibody, a.a. 861 validated for use in western blotting & IP.
Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.25 µg/mL from a representative lot detected CUX1 in 10 µg of HeLa nuclear extract.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
HeLa nuclear extract

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

ALEXA FLUOR is a registered trademark of Life Technologies

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

immunoprecipitation (IP): suitable
western blot: suitable

NP_853530

P39880

wet ice

human ... CUX1(1523)

~200 kDa and ~110 kDa observed. Uniprot describes 11 different isoforms. A full length protein has been described at 200 kDa and is proteolytically cleaved into the p150, p110, p90, and p80 isoforms. Additionally, the p75 isoform is the result of alternative splicing (Sansregret, L, et al. (2008). Gene. 412(1-2):84-94.). Uncharacterized band(s) may be observed in some cell lysates.

Stable for 1 year at 2-8°C from date of receipt.

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12 - Non Combustible Liquids

WGK 1