Anti-Osteopontin Antibody

32160702

NA.41

Osteopontin is a single-chain polypeptide. It is highly phosphorylated sialoprotein that is a prominent component of the mineralized extracellular matrices of bones and teeth. It is characterized by the presence of a polyaspartic acid sequence and sites of Ser/Thr phosphorylation that mediate hydroxyapatite binding, and a highly conserved RGD motif that mediates cell attachment/signaling. Expression of osteopontin in a variety of tissues indicates a multiplicity of functions that involve one or more of these conserved motifs. While the lack of a clear phenotype in osteopontin "knockout" mice has not established a definitive role for osteopontin in any tissue, recent studies have provided some novel and intriguing insights into the versatility of this enigmatic protein in diverse biological events, including developmental processes, wound healing, immunological responses, tumorigenesis, bone resorption, and calcification. The ability of osteopontin to stimulate cell activity through multiple receptors linked to several interactive signaling pathways can account for much of the functional diversity.

The antibody reacts with the 60 kDa osteopontin protein.

Synthetic peptide corresponding to aa 75-90 of osteopontin protein.
Epitope: aa 75-90

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Detect Osteopontin using this Anti-Osteopontin Antibody validated for use in WB, IH.

Routinely evaluated by Western Blot on mouse kidney lysate.

Western Blot Analysis:
1:500 - 1:5,000 dilution of this antibody detected osteopontin in 10 μg of mouse kidney lysate.

Serum containing 0.05% sodium azide.

Control
Mouse kidney, COS-1 and human urine lysate

rabbit

100

serum

primary antibodies

polyclonal

human, mouse

immunohistochemistry: suitable
western blot: suitable

NM_000582.2

P10451

wet ice

60 kDa

Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1