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Anti-PSAT1 Antibody
| 产品编号： | 4087762 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-PSAT1 Antibody
Phosphoserine aminotransferase, Phosphohydroxythreonine aminotransferase, PSAT
基本信息
| eCl@ss | 32160702 |
| General description【一般描述】 | Phosphoserine aminotransferase (EC 2.6.1.52; UniProt Q9Y617; also known as Phosphohydroxythreonine aminotransferase, PSAT) is encoded by the PSAT1 (also known as NLS2, PSA, PSATD) gene (Gene ID 29968) in human. PSAT1 is a class-V pyridoxal-phosphate-dependent aminotransferase family member that catalyzes the reversible conversion of 3-phosphohydroxypyruvate and to 1-phosphoserine, as well as the conversion of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to 4-phosphonooxy-L-threonine, using, in both cases, L-glutamate as an amine donor, which is converted to 2-oxoglutarate. PSAT1 is involved in serine biosynthesis from glycolytic intermediates and PSAT1 upregulation is reported in non-small cell lung cancer (NSCLC) and colon cancer cells, while PSAT1 knockdoown is shown to suppress the proliferation of MDA-MB-468 and BT-20 breast cancer cells. |
| Specificity【特异性】 | The immunogen sequence is 100% conserved in murine PSAT1 (UniProt Q99K85) and present in both spliced isoforms of human PSAT1 (Uniprot Q9Y617). |
| Immunogen【免疫原】 | Epitope: Region between the two pyridoxal phosphate-binding domains. KLH-conjugated linear peptide corresponding to a sequence within the region between the two pyridoxal phosphate-binding domains of human PSAT1. |
| Application【应用】 | This Anti-PSAT1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of PSAT1. Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected PSAT1 in A431, HeLa, HepG2, and NIH/3T3 cells. Research Sub Category Enzymes & Biochemistry Research Category Apoptosis & Cancer |
| Quality【质量】 | Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected PSAT1 in 10 µg of Jurkat cell lysate. |
| Physical form【外形】 | Affinity purified. Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rabbit (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_478059 |
| UniProt accession no.【UniProt登记号】 | Q9Y617 |
产品说明
| Target description【目标描述】 | ~40 kDa observed. 40.42 kDa (human isoform 1; Alpha) and 35.19 kDa (human isoform 2; Beta), 40.47 kDa (mouse) calculated. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
