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Anti-DARPP-32 Antibody
| 产品编号： | 4087812 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-DARPP-32 Antibody
protein phosphatase 1, regulatory (inhibitor) subunit 1B, dopamine and cAMP-regulated neuronal phosphoprotein 32, dopamine and cAMP regulated phosphoprotein, protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotei
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Dopamine- and cAMP-regulated neuronal phosphoprotein (DARP-32) is a cytoplasmic member of the protein phosphatase inhibitor 1 family. DARP-32 is a multifunctional protein kinase and protein phosphatase regulator, depending on the site of its activation. It possesses at least four sites that can be phosphorylated. Phosphorylation by PKA at Thr34 stimulates DARP-32 to act as a protein-phosphatase-1 (PP-1) inhibitor. However, phosphorylation at Thr75 will inhibit Thr34 phosphorylation. This selective, bidirectional regulation allows DARPP-32 to have a wide range of influence on downstream physiological effectors such as: ion pumps, neurotransmitter receptors, voltage-gate ion channels, and transcriptional factors. Studies suggest that it may also have a role in the Circadian System photic pathway. Overexpression of DARPP-32 has been linked with antiapoptotic mechanisms of cancer cells and has been observed in breast, colon, esophagus, gastric, and prostate cancer tissues. |
| Specificity【特异性】 | This antibody recognizes DARPP-32 near the N-terminus. |
| Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to mouse DARPP-32 near the N-terminus. |
| Application【应用】 | Anti-DARPP-32 Antibody is an antibody against DARPP-32 for use in WB & IP. Research Sub Category Neurotransmitters & Receptors Ion Channels & Transporters Western Blot (Snap i.d.) Analysis: 1 µg/mL of this antibody from a previous lot detected DARPP-32 on 10 µg of rat brain tissue lysate. Immunoprecipitaion Analysis: 10 µg of this antibody from a previous lot immunoprecipitated DARPP-32 from rat brain RIPA lysate. |
| Quality【质量】 | Evaluated by Western Blot on rat brain tissue lysate. Western Blot Analysis: 0.1 µg/mL of this antibody detected DARPP-32 on 10 µg of rat brain tissue lysate. |
| Linkage【联系】 | Replaces: AB1656 |
| Other Notes【其他说明】 | Affinity purified |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | rhesus macaque, mouse, rat |
| species reactivity (predicted by homology) | canine (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology) |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_659077 |
| UniProt accession no.【UniProt登记号】 | Q60829 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~ 32 kDa observed |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
