Anti-Chromobox protein homolog 3 Antibody

32160702

NA.41

Chromobox protein homolog 3, also known as Chromobox protein homolog 3 (HECH), Heterochromatin protein 1 homolog gamma (HP1 gamma), or Modifier 2 protein, and encoded by the gene name CBX3, is implied to be involved in transcriptional silencing in heterochromatin-like complexes. Chromobox protein homolog 3 recognizes and binds histone H3 tails methylated at ′Lys-9′, leading to epigenetic repression. Chromobox protein homolog 3 may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Chromobox protein homolog 3 may also play a role in the formation of functional kinetochore through interaction with MIS12 complex proteins. Chromobox protein homolog 3 binds directly to CHAF1A and it has been shown to interact with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2, SP100, MIS12, DSN1, CBX5 via the chromoshadow domain, POGZ, CHAMP1, and ASXL1. Chromobox protein homolog 3 is phosphorylated by PIM1. Recent studies have implied that Chromobox protein homolog 3 is phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.

KLH-conjugated linear peptide corresponding to Chromo Domain 1 of human Chromobox protein homolog 3.
Epitope: Chromo Domain 1

Research Sub Category
Nuclear Receptors
Research Category
Epigenetics & Nuclear Function
This Anti-Chromobox protein homolog 3 antibody is validated for use in WB, IC;IP for the detection of Chromobox protein homolog 3.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Chromobox protein homolog 3 in 500 µg of HeLa nuclear extract.
Immunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected Chromobox protein homolog 3 in HeLa cells.

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Chromobox protein homolog 3 in 10 µg of HeLa nuclear extract.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Concentration: Please refer to lot specific datasheet.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

bovine (based on 100% sequence homology), horse (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology), monkey (based on 100% sequence homology)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NP_009207

Q13185

wet ice

human ... CBX3(11335)

~22 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1