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Anti-LSD1 Antibody
| 产品编号： | 4087932 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-LSD1 Antibody
Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | LSD1 (lysine specific demethylase 1), also known as BHC110, is a histone demethylase that specifically demethylates Lys4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted Lys4 of histone H3, and may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. LSD1 may also demethylate Lys9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. |
| Specificity【特异性】 | This antibody recognizes LSD1. |
| Immunogen【免疫原】 | Histidine-tagged recombinant protein corresponding to full length LSD1. |
| Application【应用】 | Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated LSD1 in 0.5 mg of HeLa nuclear extract. Use Anti-LSD1 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect LSD1 also known as Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110. Research Sub Category Histones Research Category Epigenetics & Nuclear Function |
| Quality【质量】 | Evaluated by Western Blot in HeLa nuclear extract. Western Blot Analysis: 0.05 µg/mL of this antibody detected LSD1 in 10 µg of HeLa nuclear extract. |
| Linkage【联系】 | Replaces: MABE545 |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control HeLa nuclear extract |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human, mouse, rat |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_055828 |
| UniProt accession no.【UniProt登记号】 | O60341 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~110 kDa observed. Uniprot describes two isoforms at ~92 kDa and ~95 kDa due to alternative splicing. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
