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Anti-CRKL Antibody
| 产品编号： | 4087948 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-CRKL Antibody
Crk-like protein
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Crk-like protein (CRKL) is a 39 kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS. |
| Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to human CRKL. |
| Application【应用】 | Research Sub Category Apoptosis - Additional Western Blotting Analysis: 0.25 µg/mL from a representative lot detected CRKL in 10 µg of NIH3T3 and L6 cell lysate. Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated CRKL in 500 µg of Jurkat cell lysate. Detect Crk-like protein using this rabbit polyclonal antibody, Anti-CRKL Antibody validated for use in western blotting & IP. Research Category Apoptosis & Cancer |
| Quality【质量】 | Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: 0.25 µg/mL of this antibody detected CRKL in 10 µg of Jurkat cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human, rat, mouse |
| species reactivity (predicted by homology) | rhesus macaque (based on 100% sequence homology) |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_005198 |
| UniProt accession no.【UniProt登记号】 | P46109 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... CRKL(1399) |
产品说明
| Target description【目标描述】 | ~39 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
