Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody

32160702

NA.41

H1.4 is one of four variants of histone H1 (H1.2-H1.5) that is found in somatic cells. The H1 histones may function as linker proteins binding DNA via their GH domain, and enabling the wrapping of DNA around the core nucleosomal structure formed from the association of H2A, H2B, H3, and H4. In response to various stimuli, histones undergo a number of post-translational modifications (PTM) including acetylation, phoshorylation, and methylation. Previous studies have suggested that H1.4 may be phosphorylated during mitosis and interphase resulting in increased transcription of target genes by RNA polymerase I and II, and therefore this PTM on H1.4 may have implications for cell growth. H1.4 may also undergo acetylation of its N-terminal lysine residues. Often, it is the pattern of different modifications on several histone residues that alter chromatin and affect gene expression, rather than modification of single residues.

This antibody recognizes the N-terminus of Histone H1.4 acetylated at Lys26 and phosphorylated at Ser27.
Other homologies: Rat and Mouse (82% sequence homology).

KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H1.4 acetylated at Lys26 and phosphorylated at Ser27.
Epitope: N-terminus

Dot Blot Analysis: A 1:1,320 dilution from representative lot detected acetyl (Lys26) phospho (Ser27) Histone H1.4 peptides.
Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody is a rabbit polyclonal antibody for detection of acetyl (Lys26) phospho (Ser27) Histone H1.4 also known as Histone H1.4, Histone H1b & has been validated in WB, DB.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blot in colcemid treated HeLa acid extract.

Western Blot Analysis: A 1:3,000 dilution of this antibody detected acetyl (Lys26) phospho (Ser27) Histone H1.4 in 10 µg of colcemid treated HeLa acid extract.

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
Colcemid treated HeLa acid extract

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

rhesus macaque (based on 100% sequence homology), canine (based on 100% sequence homology), orangutan (based on 100% sequence homology), giant panda (based on 100% sequence homology)

dot blot: suitable
western blot: suitable

NP_005312

P10412

wet ice

~35 kDa observed. Uncharacterized bands may be observed at ~70 kDa and ~17 kDa in some acid extracts.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1