Anti-acetyl-Histone H4 Antibody

32160702

NA.41

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Acetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins.
Other species not tested, but expected to cross-react since Histone H4 is well conserved.

KLH-conjugated peptide corresponding to a.a. 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16.
Epitope: a.a. 2-19

Chromatin Immunoprecipitation:
5-10 μL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells.
Use Anti-acetyl-Histone H4 Antibody (Rabbit Polyclonal Antibody) has been published and validated in ChIP, WB, ICC to detect acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

Routinely evaluated by western blot on Tetrahymena macronuclei or acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate.

Western Blot Analysis:
1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.

Unpurified
Whole antiserum containing 0.05% sodium azide.

Control
TSA-treated Jurkat cells. For a negative control, perform a no-antibody immunoprecipitation by incubating the supernatant fraction with 60μl of Salmon Sperm DNA/Protein A Agarose- 50% Slurry. Transcriptionally unactivated DNA samples should be prepared as controls for PCR.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

serum

primary antibodies

polyclonal

eukaryotes, human, Tetrahymena sp.

Upstate^®

ChIP: suitable
immunocytochemistry: suitable
western blot: suitable

IgG

NM_175054

P62805

dry ice

10 kDa

Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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10 - Combustible liquids

WGK 1