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Anti-CDK1/CDC2 Antibody (C-Term)
产品别名
Anti-CDK1/CDC2 Antibody (C-Term)
Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Entry of all eukaryotic cells into mitosis is regulated by activation of CDC2 kinase. Activation of CDC2 is controlled at several steps including cyclin binding and phosphorylation of Thr161. However, the critical regulatory step in activating CDC2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and inhibition of CDC2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of CDC2 is carried out by the CDC25 phosphatase. Expression of CDC2 plays a critical role in cell transition through the G2/M phase. The activity of CDC2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation. Several proteins including Histone H1 and pp60src are substrates for CDC2. |
| Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to a region near the near the C-terminus of human CDK1/CDC2. |
| Application【应用】 | Research Sub Category Cell Cycle, DNA Replication & Repair Immunoprecipitation Analysis: 5 µL of this antibody immunoprecipited CDK1/CDC2 (C-terminus) from Balb/3T3/A31 cell lysate. Immunoprecipited sample was then subject to Western Blotting analysis. Research Category Epigenetics & Nuclear Function Detect Cdk1/Cdc2 with Anti-Cdk1/Cdc2 Antibody, CT (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB. |
| Quality【质量】 | Evaluated by Western Blotting in Balb/3T3/A31 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CDK1/CDC2 (C-terminus) in Balb/3T3/A31 cell lysate. |
| Physical form【外形】 | rabbit antiserum containing 0.05% sodium azide Antiserum |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
| Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | serum |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| species reactivity | mouse |
| species reactivity (predicted by homology) | human (with greater than 90% sequence homology), hamster (with greater than 90% sequence homology), rat (with greater than 90% sequence homology) |
| manufacturer/tradename | Upstate® |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG |
| NCBI accession no.【NCBI登记号】 | NP_001777 |
| UniProt accession no.【UniProt登记号】 | P06493 |
| shipped in【运输】 | dry ice |
产品说明
| Target description【目标描述】 | 34kDa |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
